Figure 5
From: Cargo binding promotes KDEL receptor clustering at the mammalian cell surface
Microtubule-assisted KDELR transport is required for cargo clustering at the cell surface.
(A) Tracking of single KDELR clusters (red) moving along the microtubule network (green). A sequence of five successive live cell imaging pictures (720 frames/h) of HeLa cells expressing mCherry-tagged Erd2.1 and GFP-tagged β-tubulin is shown. The arrows indicate an example of tubulin/KDELR signal co-localization. (B) Co-localization of GFP-tubulin and mCherry-Erd2.1. The ratio of correlated tubulin and KDELR pixels of the live cell imaging experiment is shown during 150 s. (C) KDELR/cargo cluster formation in colchicine (red) and phalloidin (inset) pre-treated cells (2.5 μM colchicine, 60 min or 10 μM phalloidin, 90 min) after incubation with 160 μg/ml eGFP-RTAHDEL. Temporal evolution of the accumulated KDELR/cargo is compared with the untreated control cells. The inset shows a comparison between untreated (solid line) and phalloidin-treated HeLa cells (dashed line).
