Figure 2: PP242 induces the formation of massive vacuoles and increases LC3-II levels in NSCLC cells.
From: Inhibition of JNK-mediated autophagy enhances NSCLC cell sensitivity to mTORC1/2 inhibitors
(a,b) H460 and A549 cells were treated with the indicated concentrations of PP242 for 24 h. (c) H460 and A549 cells were treated with 10 μM PP242 for the indicated amount of time. (a) Changes in cellular morphology were observed using an inverted microscope (magnification = 400×, scale bar = 5 mm). (b,c) The percentage of vacuolated cells was calculated from 3 independent images. The data are presented as the mean ± standard deviation. (d) H460 cells were treated with vehicle or 10 μM PP242 for 16 h, stained with acridine orange, and red fluorescence (FL3 channel) was quantified using FACS analysis. (e) H460 cells treated with vehicle or 10 μM PP242 for 8 h were stained for LC3 antibody after para-formaldehyde fixation. (f) H460 cells were treated with 10 μM PP242 for the indicated amount of time. Protein levels were estimated using western blot analysis. β-actin was used as a loading control. The blot shown is representative of 2 independent experiments.
