Figure 3: PP242 induces autophagic flux in NSCLC cells.
From: Inhibition of JNK-mediated autophagy enhances NSCLC cell sensitivity to mTORC1/2 inhibitors
(a–d,g,h) H460 cells were pretreated with 50 mM 3-MA, 20 μM CHX or 100 nM BAF for 1 h prior to treatment with 10 μM PP242 for 6 h. (e,f) H460 cells were transfected with control, Atg5, or Atg7 siRNAs for 16 h, and subsequently treated with 10 μM PP242 for 8 h. (a,b,g) Changes in cellular morphology were observed using an inverted microscope (magnification = 400×, scale bar = 5 mm). (c–f,h) The levels of the indicated proteins were estimated using western blot analysis. β-actin was used as a loading control. The blot shown is representative of 2 independent experiments.
