Figure 3
Detection of anti-BmNPV activity in BmAtlastin-n overexpressed BmN-SWU1 cells.
(A) The level of transcription of BmAtlastin-n in BmAtlastin-n overexpressing BmN-SWU1 cells and empty vector control cells was determined using qRT-PCR (*P < 0.05, **P < 0.01). (B) Transfected BmAtlastin-n BmN-SWU1 cells and control cells without BmAtlastin-n transfected were incubated with v39Kprm-EGFP BmNPV. Infected cells (GFP positive) were examined using fluorescence microscopy at 12 h, 24 h and 48 h post infection. (C) BmNPV infection rates in BmAtlastin-n-OE BmN-SWU1 cells and control cells were analyzed by flow cytometry at 24 h and 48 h post infection. GFP-negative cells (GFP−) were uninfected and GFP-positive cells (GFP+) were infected. (D) Proliferation of BmNPV was assessed using qRT-PCR by analyzing relative transcription levels of VP39 at 12 h, 24 h and 48 h post infection (*P < 0.05, **P < 0.01). The rpl3 gene served as an internal control. (E) Western blots of expression of viral late 39 K protein in BmAtlastin-n overexpression BmN-SWU1 cells and pIZ/V5-His-vector-transfected control cells. Tubulin protein served as the loading control.
