Figure 2
OBP-301 enhances chemotherapy-induced apoptosis and DNA damage.
Western blot analysis was performed under the same experimental conditions. (a) Expression of PARP and cleaved PARP (C-PARP) proteins in SaOS-2 and MNNG/HOS cells treated with chemotherapeutic agents (CDDP, DOX) at the indicated doses. (b) Expression of PARP, C-PARP and adenoviral E1A proteins in SaOS-2 and MNNG/HOS cells treated with OBP-301 at the indicated MOIs. (c) Expression of PARP, C-PARP, γ-H2AX and adenoviral E1A proteins in SaOS-2 and MNNG/HOS cells treated with OBP-301 and chemotherapeutic agents (CDDP, DOX). β-actin was used as a loading control. (d) The percentage of apoptotic population was analyzed by TUNEL assay in SaOS-2 and MNNG/HOS cells after treatment with control, CDDP, DOX, OBP-301, or the combination of OBP-301 and chemotherapeutic agents. Cells were infected with OBP-301 at doses of 10 or 50 MOI, respectively. Two days after OBP-301 infection, cells were treated with cisplatin (CDDP) or doxorubicin (DOX) at 5 or 1 μg/ml, respectively, for 24 hours. Data are expressed as mean values ± SD (n = 5). Statistical significance (*) was defined as P < 0.05.
