Figure 3
OBP-301-mediated enhancement of apoptosis in combination with chemotherapy.
(a) The cell-cycle state was analyzed by flow cytometry in SaOS-2 and MNNG/HOS cells stained with propidium iodide after treatment with mock (control), CDDP, DOX, OBP-301, or the combination of OBP-301 and chemotherapeutic agents (CDDP, DOX). SaOS-2 and MNNG/HOS cells were infected with OBP-301 at doses of 10 or 50 MOI, respectively. Two days after OBP-301 infection, cells were treated with cisplatin (CDDP) or doxorubicin (DOX) at 5 or 1 μg/ml, respectively, for 24 hours. Representative cell-cycle data are shown. (b) The percentage of sub-G1 population was analyzed using flow cytometry in SaOS-2 and MNNG/HOS cells after treatment with control, CDDP, DOX, OBP-301, or the combination of OBP-301 and chemotherapeutic agents. Data are expressed as mean values ± SD (n = 3). Statistical significance (*) was defined as P < 0.05.
