Table 1 Expression of Agouti Signaling Peptide (ASIP) in rainbow trout skin analyzed after 14 days with cortisol implants.

From: A novel role for pigment genes in the stress response in rainbow trout (Oncorhynchus mykiss)

Treatment

Individual no.

S18

β-actin

ASIP

Vehicle

1

−10.21

−11.85

−22.83

Vehicle

2

−10.17

−11.76

−20.85

Vehicle

3

−10.31

−11.72

<−23

Vehicle

4

−10.36

−11.85

<−23

Vehicle

5

−10.19

−12.00

−22.49

Vehicle

6

−10.13

−12.06

<−23

Vehicle

7

−10.11

−11.96

−21.96

Vehicle

8

−10.07

−12.08

<−23

Cortisol

1

−10.09

−11.62

−13.75

Cortisol

2

−10.15

−11.60

−13.57

Cortisol

3

−10.20

−11.66

−13.71

Cortisol

4

−10.12

−11.40

−14.14

Cortisol

5

−10.28

−11.69

−14.03

Cortisol

6

−10.16

−11.66

−13.95

Cortisol

7

−10.17

−11.70

−14.04

Cortisol

8

−10.15

−11.62

−13.95

  1. log EC50 values from real competitive PCR are given. ASIP expression was below detection limit for reliable quantification using rcPCR in n = 8 untreated controls (log EC50 < −19), but clearly detectable in n = 8 cortisol treated fish (Chi-Sq = 12.25, p < 0.001). No such effect was seen on two reference genes S18 and β-actin.