Figure 4
Detection of HPV antibodies in human sera.
Using our test configuration we (a) immobilized recombinant HPV16 E7 protein and specifically detected HPV16 E7 antibodies from serum samples over a series of dilutions (1:4, 1:20 and 1:200). Representative images from each corresponding dilution as well as integrated ramp plots demonstrate assay specificity and background or off-target signal levels. To determine intra and inter assay variability we screened serum positive for HPV16 E7 antibodies and whole human IgG positive control in duplicate over four consecutive days. (b) Calculated values of intra assay standard error of the mean and (c) inter assay coefficient of variance.
