Figure 9
From: Synergistic Effect of H2O2 and NO2 in Cell Death Induced by Cold Atmospheric He Plasma
The concentrations of H2O2, NO2− and NO3− in plasma-treated PBS(Ca2+/Mg2+) are decreased in the presence of a shielding gas of pure oxygen.
Three milliliters of PBS(Ca2+/Mg2+) were set per well in 12 well plates and exposed to He plasma at a gas flow of 100 sccm for the indicated period of times and at an output voltage of 5.5 kV. The treatments were performed in the presence (with O2) or absence (no O2) of a shielding gas of oxygen at a gas flow of 5 slm. (A) The concentration of plasma-induced H2O2 for each time point was determined by using the Na3VO4 assay and the TiOSO4 assay. (B) The concentrations of NO2− and NO3− after 4 min of plasma treatment were determined by the Griess assay.
