Figure 5
Crystal structure of CGL1.
Crystal structures are shown in ribbon mode. (A) CGL1/FREE: CGL1 purified from Crassostrea gigas, dialyzed against TBS and concentrated to 5 mg/mL; (B) CGL1/MAN: CGL1 purified from C. gigas, undialyzed and concentrated to 10 mg/mL; and (C) CGL1/MAN2: CGL1 purified after heterogenous expression in Escherichia coli, undialyzed and concentrated to 10 mg/mL with 100 mM mannose. Each protomer is shown in a different color. Mannose molecules that are bound to CGL1 are shown as orange stick figures. Each protomer of the dimer structure of CGL1/FREE and CGL1/MAN is related by 2-fold crystallographic symmetry. The dimer structure of MAN2 is an asymmetric unit. (D) Superposed structures of CGL1/FREE (blue) and CGL1/MAN2 (red). Side (E) and top (F) views of CGL1 domains are shown as a ribbon diagram. The numbering in panel E is the same as in Supplementary Fig. S8. Mannose molecules bound to sites A and B are shown as red and blue stick figures, respectively. (G) Superposed structures of domains A and B, which are related according to pseudo-2-fold symmetry. Each domain is colored differently.
