Table 1 Plasmids used in this study.
Plasmid name | Description | Source |
|---|---|---|
hCOG1 1-702-3Myc | Human COG1(aa1-702) in pCMV-3Myc | This study |
hCOG1-3Myc | Human Cog1siRES in pCMV-3Myc | Willett et al.6 |
mCOG3-3Myc | Mouse COG3 in pCMV-3Myc | Willett et al.6 |
hCOG7-3Myc | Human COG7 in pCMV-3Myc | Ha et al.15 |
hCOG6-3Myc/hCOG8-3Myc | Human COG6-3Myc and COG8-3Myc in MultiLabel vectors (ATG:biosynthetics GmbH) | Willett et al.6 |
hCOG5-3Myc/hCOG7-3Myc | Human COG5-3Myc and COG7-3Myc in MultiLabel vectors (ATG:biosynthetics GmbH) | Willett et al.6 |
hCOG2-Strep-3Myc/hCOG4-3Myc/hCOG1-3Myc | Human COG1-3Myc, COG2Streptag-3Myc, and COG4-3Myc in MultiLabel vectors (ATG:biosynthetics GmbH) | Willett et al.6 |
hCOG1-(HA-GS-) or (GFP-GS-) TEV-hCOG8-3Myc | Human COG1-siRES, COG8-siRES and TEV protease recognition sequence in pCMV-3Myc | This study |
hCOG1-TEV-3Myc | Human COG1-siRES and TEV protease recognition sequence in pCMV-3Myc | This study |
hCOG8sir-mCh-ActA | Human COG8siRES in pmCherry-ActA | Willett et al.22 |
hCOG8sir-GFP-ActA | Human COG8siRES in pGFP-ActA | This study |
hCOG8sir 1–406 GFP-ActA | Human COG8siRES (aa1–406) in pGFP-ActA | This study |
COG8sir 1–136 GFP-ActA | Human COG8siRES (aa1–136) in pGFP-ActA | This study |
hCOG8sir Δ8–406 GFP-ActA | Human COG8siRES (aaΔ8–406) in pGFP-ActA | This study |
hCOG8sir Δ8-184-3Myc | Human COG8siRES (aaΔ8-184) in pCMV-3Myc | Willett et al.22 |
GFP-hGS15 | Human GS15 in pEGFP-C1 | Shestakova et al.17 |
GFP-P115 | P115 in pEGFP-C2 | E. Sztul |
GFP-ActA | A. Linstedt | |
GFP-hCOG6 | Human COG6 in pGFP-C1 | Pokrovskaya et al.9 |
GFP-mCOG3 | Mouse COG3 in pEGFP-C1 | Zolov et al.40 |
GFP-hCOG5 | Human COG5 in pEGFP-C1 | Ha et al.15 |
GFP | pEGFP-C1 | |
His-hGS15-GST | Human GS15 cytoplasmic domain (aa 1–86) with N-terminal polyhistidine tag and C-terminal GST tag in pET28a | This study |
GST | pGEX4T-1 |
