Figure 1: Immunofluorescence and immunoprecipitation analyses of PML’s influence on PER2 and BMAL1 interaction.
From: Interactive Organization of the Circadian Core Regulators PER2, BMAL1, CLOCK and PML
Immunofluorescence analysis and DAPI staining of Pml−/− MEF transfected with plasmids expressing (a) PER2 (red) and HA-BMAL1 (green). (b) K487R PML, PER2 (red) and HA-BMAL1 (green). (c) PML, PER2 (red) and HA-BMAL1 (green). (d) WT MEF cells transfected with plasmids expressing PER2 (red) and HA-BMAL1 (green). We observed more than 20 cells and pictures are representative images. (e) Left: western analysis of cytosol and nuclear fractions for wild type and Pml−/− MEF transfected with plasmid expressing PER2 and BMAL1 using the respective antibodies for PER2 and BMAL1. Right: statistical analysis of cytosol (C)/nuclear fractions (N) ratio of indicated proteins. WT MEF cells were set to 1.0. Bars: SEM. P < 0.01. N.S.: not significant. n > 3. (f) Western analysis of IP of Pml−/− MEF transfected with the respective plasmids pcDNA, Wt Pml and K487R Pml using the appropriate antibodies for PER2 and HA (n = 2).
