Figure 3 | Scientific Reports

Figure 3

From: G-protein-coupled receptor kinase 2 terminates G-protein-coupled receptor function in steroid hormone 20-hydroxyecdysone signaling

Figure 3

GRK2 suppressed 20E-induced apoptosis.

(A) Hematoxylin and eosin (HE) staining showing midgut remodeling 60 h after dsGRK2 injection (500 ng dsRNA/larva). Lu, midgut lumen; LM, larval midgut; IM, imaginal midgut. The bar represents 20 μm. (B) The cellular morphology (observed by Nikon eclipse TS100 microscopy) in GRK2 knockdown cells after incubation with 20E for 72 h (1 μg of dsGRK2 for 24 h and then 1 μM 20E for 72 h); dsGFP + 20E is the control. Ap: apoptotic vesicles. (C) Caspase-3 activity in GRK2 knockdown cells (1 μg dsGRK2, 1 μM 20E for 72 h); dsGFP + 20E is the control. Green fluorescence indicates Caspase-3 activity. Blue indicates nuclear stained by 4-6-diamidino-2-phenylindole dihydrochloride (DAPI). Merge is the overlapped green and blue. (D) Quantification of caspase-3-stained cells in (C) was performed using Image pro plus. The bars indicate the means + SD of three independent experiments.

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