Table 1 Primers used in PCR.
Primer name | (5′ → 3′) nucleotide sequence |
|---|---|
GRK2-F1- EcoRI | tactcagaattcaaaaagattgttctgcca |
GRK2-R1-Xho I | tactcactcgagtcagccgatgatgcggtg |
GRK2-RNAi-F | gcgtaatacgactcactatagggcgactttagcgtgcaccg |
GRK2-RNAi-R | gcgtaatacgactcactatagggtagcccgtgccttttgac |
GFP-RNAi-F | gcgtaatacgactcactataggtggtcccaattctcgtggaac |
GFP-RNAi-R | gcgtaatacgactcactataggagctggagacaactcctcacg |
GRK2-G/YFP-F | tactcacaattggatggcggacctcgaggct |
GRK2-G/YFP-R | tactcaggcgcgccgagttgctcccgttgggcgc |
GRK2-T672A-GFP-F | atctacggcgcggacggcgccaa |
GRK2-T672A-GFP-R | ttggcgccgtccgcgccgtagat |
GRK2-S680A-GFP-F | aaggacgccatggcgctggtgcg |
GRK2-S680A-GFP-R | cgcaccagcgccatggcgtcctt |
GPCR-2-C/YFP-F | tactcagagctcatgattacattcataacagtg |
GPCR-2-C/YFP-R | tactcacaattgggaggctgtttgatgttgagcga |
GRK2-QRT-F | aaagtgacaaatacacgaggtt |
GRK2-QRT-F | cttggacctgatgaacgg |
Actin-QRT-F | cctggtattctgaccgtatgc |
Actin-QRT-R | ctgttggaaggtggagagggaa |
EcR-B1-QRT-F | aattgcccgtcagtacga |
EcR-B1-QRT-R | tgagcttctcattgagga |
USP1-QRT-F | ggtcctgacagcaatgtt |
USP1-QRT-R | ttccagctccagctgactgaag |
HR3-QRT-F | tcaagcacctcaacagcagcccta |
HR3-QRT-R | gactttgctgatgtcaccctccgc |
Br-QRT-F | ggtgactgtccttactgcggcat |
Br-QRT-R | ttaattcctttgaccatgact |
Kr-h1-F | gcggtgggcctccacgtgtcgaacg |
Kr-h1-R | cgttcgacacgtggaggcccaccgc |
