Table 1 The average expression levels (TPM) in V. volvacea and the predicted function of the five newly selected ICGs.

From: Identification of novel and robust internal control genes from Volvariella volvacea that are suitable for RT-qPCR in filamentous fungi

Gene symbol

Mean TPM

Gene name

Pfam annotation

KOG annotation

Function

L-asp

6.30

L-asparaginase

Asparaginase (PF00710)

Asparaginase; Amino acid transport and metabolism

Catalyze the hydrolysis of asparagine

Ras

7.88

Ras protein

Ras family (PF00071)

Ras-related small GTPase; General function prediction only

Regulate signaling networks involved in actin organization, cell proliferation, differentiation, vesicular trafficking

SPRYp

61.76

Endosomal SPRY domain protein

SPRY domain (PF00622)

SPRY domain-containing proteins; General function prediction only

Be involved in cargo recognition and nutrient transport as a component of the endosome-vacuole trafficking pathway

Vps26

89.46

Vacuolar protein sorting-associated protein 26

Vacuolar protein sorting-associated protein 26 (PF03643)

Membrane coat complex Retromer, subunit VPS26; Intracellular trafficking, secretion and vesicular transport

Participate in protein trafficking to the vacuole and mediate cargo selection

MSF1

156.28

MSF1 domain containing protein

PRELI-like family (PF04707)

Member of the intra-mitochondrial sorting protein family; Intracellular trafficking, secretion and vesicular transport

Be involved in intra-mitochondrial protein sorting; maintain mitochondrial electron transport chain and respiratory competency