Table 2 Descriptions of fourteen candidate ICGs in V. volvacea and parameters derived from RT-qPCR analysis.
Gene symbol | Gene name | Accession number | Primer sequences (5′–3′) | Product size (bp) | PCR efficiency (100%)a | Annealing temperature (°C) |
|---|---|---|---|---|---|---|
18S | 18S ribosomal RNA | KF471312 | F: TCTTGTGAAACTCTGTCGTGCTGGG | 186 | 1.87 | 66 |
R: TTGCCCACACCCCAAAGCTAATTCG | ||||||
ACTB | β-actin | KF528321 | F: AGCTCTCTTCCAACCTGCCTTCTTG | 216 | 1.98 | 62 |
R: GCGACAATCTTAACCTTCATGCTTGC | ||||||
CYP | Cyclophilin | KF528322 | F: GATCCCAATTCTTCGTCACCACTG | 125 | 1.97 | 63 |
R: ACCACTTGCAGATCCAAAGCCCTCG | ||||||
GAPDH | Glyceraldehyde 3-phosphate dehydrogenase | KF528323 | F: ATTGGCGTGGTGGTCGTAG | 142 | 1.95 | 60 |
R: ACGGAAACATCAAGGGTAGGG | ||||||
HH3 | Histone H3 | KF528324 | F: GTGAAATCAGACGTTACCAGAAGT | 219 | 2.03 | 59 |
R: TGGGTTGGATAGTGACACGCTTAG | ||||||
α-TUB | α-tubulin | KF528325 | F: ACTTGGTTCCTTTCCCTCGTATTCAC | 169 | 2.02 | 61 |
R: GCCATGTATTTGCCTTCCCTAG | ||||||
β-TUB1 | β-tubulin 1 | KF528326 | F: GATTCCCAACAACGTCCTCACC | 204 | 1.90 | 61 |
R: TCAGCTTCCGTGAACTCCATCTC | ||||||
β-TUB2 | β-tubulin 2 | KF528327 | F: GAACTCAAGAGACATTCGCTGCC | 164 | 1.94 | 63 |
R: TTCACCATCCTCCACAGACGCTTC | ||||||
UBQ | Ubiquitin | KF528328 | F: GGAAAGACGATCACCTTGGAG | 191 | 1.95 | 60 |
R: AGGCGAAGAACAAGATGAAGAGTG | ||||||
L-asp | L-asparaginase | KF528333 | F: TTCGTGTCGCTCTCTCTCCAG | 141 | 2.00 | 59 |
R: GTATTGACTGTTTGTGAGGCTTGT | ||||||
Ras | Ras protein | KF528332 | F: ACATACGAGAGAGAAGTTTCCAAG | 181 | 2.13 | 59 |
R: TCTCCTCCTTCTGTGGCTTGAC | ||||||
SPRYp | Endosomal SPRY domain protein | KF528330 | F: CTCCTCCTCCAATAACCCCCATC | 181 | 2.03 | 62 |
R: TCAGAGTCTATTCCTTGCCTATCCG | ||||||
Vps26 | Vacuolar protein sorting associated protein 26 | KF528331 | F: CGATTCGACTGTTCCTTGGTGG | 127 | 1.99 | 61 |
R: TGCTTGAAATATCGGCGGTTCTC | ||||||
MSF1 | MSF1 domain containing protein | KF528329 | F: CAGATGGACTAGAGAAGTGGTTAG | 136 | 1.94 | 59 |
R: ATCTCTTATGAAGGTGCGTGTG |
