Figure 4

Testing the effectiveness of the selected model 5 (hierarchical mechanism 1).
(A) Simulated (solid lines) and experimental data (dots) for ISRE activation with stimulation by intracellular (IC) poly (I:C). (B) Simulated and experimental data for the dose-responses of ISRE activation by IC poly (I:C) at various dosages (ranging from 0 μg/mL to 10 μg/mL). (C) Cross validation of the estimated parameters using the bootstrap approach. The coefficient of variation (CV) was used to evaluate the identifiability of the estimated parameters. (D) Parameter sensitivity analysis. (E) Testing the parameterized model using an independent set of luciferase assay data.