Figure 5: DTBIA for the detection of CaLas in sweet orange petioles using rabbit polyclonal antibody raised against the major outer membrane protein OmpA.

The primary antibody (1:5000 dilution) was detected with Goat anti-rabbit secondary Pab diluted 1:50,000. (A–E) CaLas B430 in different sections of petioles. (F) Healthy Sweet orange. Detection was with NBT/BCIP.