Table 1 Plasmids used in this study.

pACYC184

origin p15A (15 copies per cell), Cm^R, Tetracycline^R

pACYC184ts

origin p15A (15 copies per cell), Cm^R, with a deletion in the tetracycline resistance gene

phfq

pACYC184-derivative carrying Y. enterocolitica hfq

pYopH-L-Bla

pACYC184 derivative carrying the promoter and coding sequence of the yopH gene (without stop codon) fused in frame through linker (L) peptide (GGGGS)₃ to TEM-1 β-lactamase gene bla, Cm^R

pFX-P

origin RSF1010 (10-12 copies per cell), Sp^R, gfp gene without start codon, used to generate translational fusions

pFX-0

pFX-P carrying a promoter-less complete gfp gene, used as a negative control, Sp^R

pFX-1

pFX-P carrying a complete gfp gene under control of the P_lac promoter

pFX-2

pFX-P carrying a complete gfp gene under control of the P_LtetO-1 promoter

pFX-ail

pFX-P carrying 380 bp upstream of ail start codon, and 9 codons of ail fused in frame with gfp

pFX-PtetO-ail

pFX-P carrying the P_LtetO-1 promoter, 59 bp upstream of ail start codon, and 9 codons of ail fused in frame with gfp

pFX-ompX

pFX-P carrying 402 bp upstream of ompX start codon, and 5 codons of ompX fused in frame with gfp

pFX-Plac-ompX

pFX-P carrying the P_lac promoter, 44 bp upstream of ompX start codon, and 5 codons of ompX fused in frame with gfp

pFX-invA

pFX-P carrying 494 bp upstream of invA start codon, and 4 codons of invA fused in frame with gfp

pFX-Plac-invA

pFX-P carrying the P_lac promoter, 121 bp upstream of invA start codon, and 4 codons of invA fused in frame with gfp

pFX-yadA

pFX-P carrying 686 bp upstream of yadA start codon, and 16 codons of yadA fused in frame with gfp

pFX-Plac-yadA

pFX-P carrying the P_lac promoter, 264 bp upstream of yadA start codon, and 16 codons of yadA fused in frame with gfp

pFX-rovA

pFX-P carrying 634 bp upstream of rovA start codon, and 6 codons of rovA fused in frame with gfp

pFX-ompR

pFX-P carrying 188 nucleotides upstream of ompR start codon, and 4 codons of ompR fused in frame with gfp

pFX-phoP

pFX-P carrying 860 bp upstream of phoP start codon, and 5 codons of phoP fused in frame with gfp

pFX-myfA

pFX-P carrying 173 bp upstream of myfA start codon, and 7 codons of myfA fused in frame with gfp

pFX-plac-myfA

pFX-P carrying the P_lac promoter, 99 bp upstream of myfA start codon, and 7 codons of myfA fused in frame with gfp