Figure 2 | Scientific Reports

Figure 2

From: Nucleotide-binding oligomerization domain-containing protein 2 prompts potent inflammatory stimuli during Neospora caninum infection

Figure 2

Nod2 leads to pro-inflammatory responses in macrophages during N. caninum infection.

The levels of IL-6 (A,D), TNF-α (B,E) and IL-10 (C,F) were measured by ELISA in cell-free supernatants of naïve WT and Nod2−/− BMDMs, or infected with N. caninum tachyzoites (MOI 0.2), at 8 h (A–C) and 24 h (D–F) post-infection. Arginase I (G) and iNOS (H) expression were quantified, by Western Blot, in lysates of WT and Nod2−/− BMDMs, 48 hours post-infection with N. caninum tachyzoites (MOI 0.2). All results are shown as means of triplicate cultures of BMDMs and the data are representative of at least two independent experiments. Error bars indicate mean ± SEM. #P ≤ 0.05 compared to the medium; *P ≤ 0.05, **P ≤ 0.01 compared to WT group. ND = not detected.

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