Figure 3 | Scientific Reports

Figure 3

From: Nucleotide-binding oligomerization domain-containing protein 2 prompts potent inflammatory stimuli during Neospora caninum infection

Figure 3

Nod2-dependent response is required for the restriction of Neospora caninum replication.

BMDMs from WT and Nod2−/− mice were infected with DDAO-stained N. caninum tachyzoites (MOI 0.2) and evaluated for the percentage of infected cells (A) and the mean fluorescence (B), after 24 h of infection, by flow cytometry. Results are shown as the mean of nine biological replicates and are representative of two independent experiments. Nod2−/− and WT mice were infected with sub-lethal doses of N. caninum tachyzoites (1.107 tachyzoites, i.p. route) and the survival was evaluated for 30 days (n = 8 mice per group, (C)). In a parallel experiment, peritoneal exudate cells (D) and pancreas (E) of mice submitted to sub-lethal infections were checked for parasite load by quantitative PCR after 5 days of infection (n = 5 mice per group). *Statistical difference (*P ≤ 0.05) between WT and Nod2−/− mice.

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