Figure 1 | Scientific Reports

Figure 1

From: Genome-wide analysis of HIF-2α chromatin binding sites under normoxia in human bronchial epithelial cells (BEAS-2B) suggests its diverse functions

Figure 1

Expression of pluripotency related markers and a sphere culture of BEAS-2B under normoxia.

(a) HIF-2α expression was detected in the nucleus of BEAS-2B under normoxia by western blotting. Beta tubulin and TATA binding protein (TBP) were used as protein markers for the cytosol and nucleus fraction, respectively. (b) Western blotting for Oct-4. (c) For knockdown of HIF-2α, BEAS-2B cells were transfected with shRNA targeting HIF-2α and sh-Luc was used as control. (d) Western blotting of Nanog and a culture of BEAS-2B containing floating spheres. Representative result from two or three experiments was presented.

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