Figure 3 | Scientific Reports

Figure 3

From: Genome-wide analysis of HIF-2α chromatin binding sites under normoxia in human bronchial epithelial cells (BEAS-2B) suggests its diverse functions

Figure 3

Chromatin enrichment of HIF-2α analyzed by using ChIP-PCR.

(a) Representative enrichments of HIF-2α provided by ChIP-Seq analysis were further verified using semiquantitative PCR. Data are presented as signal relative to that of input DNA (100%). *P < 0.05 and **P < 0.01 vs. negative control. (b) The confirmed enrichments were further subjected to hypoxia mimetic treatment using CoCl2 (150 μM). Antibody against HIF-2α or P300 was used with the same primer pair. Signal of semiquantitative PCR relative to that of input DNA was used to calculate the relative fold binding between hypoxia and normoxia. All values were estimated by using image J (mean ± SD, n = 3).

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