Figure 6
Hit validation and screening of ChemBridge compounds.
(A) Hits from the Prestwick, Asinex Protein-Protein Interaction (PPI) and Malaria Box library screens were re-screened at 10 μM together with selected compounds from the ChemBridge drug library (100 μM), DMSO (0.1%) and Heparin (10 mg/mL) controls. Rosetting FCR3S1.2R parasites cultured in A+ erythrocytes were stained and treated for 2 h with test 10 μM compounds before flow cytometry analyses. (B) Compound properties of MMV006764 from ChEMBL database are tabled here including molecular weight (MW), charge, Lipinsky rule of 5 violations (#Ro5), effective concentration 50% (EC50) and inhibitory concentration 50% (IC50) values.
