Figure 7
Validation of MMV006764 rosette disruption activity.
Rosetting FCR3S1.2R parasites cultured in blood group A erythrocytes were treated for 2 h with various concentrations of (A) MMV006764 or (B) Heparin and the samples split for microscopic enumeration or (C) flow cytometry for correlation. (Between 150–300 late-stage parasites were counted in each microscopy sample, *P < 0.05, **P < 0.01, ****P < 0.0001, N = 3) (D) Cultures of rosetting FCR3S1.2R, PAvarO and R29 grown in blood group O+ or A+ erythrocytes were stained and treated with different concentrations of MMV006764 for 2 h before flow cytometry (**all points in box compared to untreated P < 0.01, N = 6, details of P-values in Supplementary Table S4). Rosettes of FCR3S1.2R grown in (E) blood group O+ or (F) A+ erythrocytes were dispersed by trituration and allowed to reform spontaneously in MCM, with or without 50 μM of MMV006764, MMV006656, AG-205/40776006 and ST017207, or 1 mg/ml of heparin.
