Figure 4 | Scientific Reports

Figure 4

From: Molecular cloning and functional characterization of the sex-determination gene doublesex in the sexually dimorphic broad-horned beetle Gnatocerus cornutus (Coleoptera, Tenebrionidae)

Figure 4

Predicted gene model and expression patterns of Gcdsx splicing variants.

(A) Schematic gene model of identified Gcdsx splicing variants. Boxes indicate exons. White boxes indicate UTRs. Black and dark grey boxes indicate DM and OD2 domains, respectively. Light grey boxes indicate other translated portions of the proteins. Arrows indicate the primer positions used in expression analyses via RT-PCR. (B) Expression pattern of each splicing variant of Gcdsx during postembryonic development in both sexes. P1-P2 pair can amplify Gcdsx-M, Gcdsx-FS and Gcdsx-FL. P3-P4 pair can amplify Gcdsx-C1 and Gcdsx-C2. Gcrp49 was used as internal control.

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