Table 1 Degenerate and RACE-PCR primers.

From: Molecular cloning and functional characterization of the sex-determination gene doublesex in the sexually dimorphic broad-horned beetle Gnatocerus cornutus (Coleoptera, Tenebrionidae)

Gcdsx-degenerate-F

5′-AAYTGYGCIMGITGYMGIAAYCA-3′

Gcdsx-degenerate-R

5′-TACATIARIGGCATCATYTCCCA-3′

Gcrp49-F

5′-ACIAARMAITTYATIMGICA-3′

Gcrp49-R

5′-TGIGCIATYTCISCRCARTA-3′

5′-RACE-GSP

5′-GACTCTTCTGCAGGACATGCGGGTCTAT-3′

5′-RACE-NGSP

5′-ACTTGCAGTACCTCTTGTGGCCCTTGA-3′

3′-RACE-GSP

5′-CTCAAGGGCCACAAGAGGTACTGCAAG-3′

3′-RACE-NGSP

5′-GTCCTGCAGAAGAGTCCTTCGCCGATAC-3′

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