Table 2 Primers for dsRNA synthesis.

From: Molecular cloning and functional characterization of the sex-determination gene doublesex in the sexually dimorphic broad-horned beetle Gnatocerus cornutus (Coleoptera, Tenebrionidae)

Gcdsx-exon1,2-F

5′-AAYTGYGCIMGITGYMGIAAYCA-3′

Gcdsx-exon1,2-R

5′-TACATIARIGGCATCATYTCCCA-3′

Gcdsx-exon4-F

5′-CCAAGAAAGGAGAATCAACGG-3′

Gcdsx-exon4-R

5′-CAACAAAGTGACGTCGCCGCTGGG-3′

Gcdsx-exon5-F

5′-TGCACAAGAACTCAACAAGAAG-3′

Gcdsx-exon5-R

5′-TTGGGACAAACGCTCCAGT-3′

Gcdsx-exon8-F

5′-TTCCAAACCGTGAATCACAA-3′

Gcdsx-exon8-R

5′-CTTGGAGCCCACTCTGAATC-3′

  1. Gcdsx-exon1,2-F and R are identical to Gcdsx-denegenerate-F and R.
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