Figure 2: LysGH15 induced specific IgG antibodies. | Scientific Reports

Figure 2: LysGH15 induced specific IgG antibodies.

From: LysGH15 kills Staphylococcus aureus without being affected by the humoral immune response or inducing inflammation

Figure 2

Serum samples from immunized mice and control mice were collected every week for 10 weeks, and the titres (A) and concentrations of IgG, IgM and IgE isotypes (B) were measured using ELISA. (C) Western blotting assay of LysGH15. Mouse serum at 3 weeks post-immunization was used as the primary antibody (dilution 1:500), and HRP-labeled anti-mouse IgG antibody was used as the secondary antibody. The left lane shows the results for the LysGH15-injected mouse. The right lane shows the LysGH15 buffer-injected mouse. (n = 6 mice per group per experiment). *P < 0.05 compared with unimmunized mice. The data are representative of three experiments.

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