Figure 4: The effect of PPARγ2 overexpression on wild type and SIRT1 transgenic mice. | Scientific Reports

Figure 4: The effect of PPARγ2 overexpression on wild type and SIRT1 transgenic mice.

From: Suppression of PPARγ-mediated monoacylglycerol O-acyltransferase 1 expression ameliorates alcoholic hepatic steatosis

Figure 4

(a) Schedule of PPARγ2 overexpression. Wild type and SIRT1 transgenic mice were injected with Ad-PPARγ2 or an Ad-GFP via the tail vein, fed a chow diet for 1 week, and then sacrificed. (b) H&E staining performed on liver sections of wild type and SIRT1 transgenic mice. (c) Hepatic TG content in wild type and SIRT1 transgenic mice infected with Ad-GFP or Ad-PPARγ2. (d) Real-time PCR analysis of PPARγ target genes and lipogenic genes in wild type and SIRT1 transgenic mice. (e) Immunofluorescence staining for adipose differentiation-related protein (ADRP, green) in mouse primary hepatocytes. Nuclei were stained with DAPI and fluorescence was visualized by confocal microscopy. Scale bar = 50 μm. Data in c and d represent the mean ± SD. *P < 0.05, **P < 0.01.

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