Figure 6: Ethanol metabolism in binge drinking leads to the activation of SREBP1c and ChREBP. | Scientific Reports

Figure 6: Ethanol metabolism in binge drinking leads to the activation of SREBP1c and ChREBP.

From: Suppression of PPARγ-mediated monoacylglycerol O-acyltransferase 1 expression ameliorates alcoholic hepatic steatosis

Figure 6

(a) Schedule of binge drinking experiment. B6 mice were fasted for 4 h before receiving two gavages of equivalent calories of ethanol at 3.5 g/kg or dextrin-maltose (DM). Mice were sacrificed 6 h later. (b) NAD+/NADH ratio examined in DM and ethanol-fed mice group. (n = 6 per group) (c) Western blot analysis showing SIRT1 expression in DM and ethanol-fed mice group. (n = 4 per group) (d) PPARγ acetylation in DM and ethanol-fed mice group. (n = 3 per group) (e,f) Real-time PCR analysis showing expression of PPARγ, SREBP1c, ChREBP, and their target genes. (n = 6). Data represent the mean ± SD. **P < 0.01.

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