Figure 3 | Scientific Reports

Figure 3

From: Human induced pluripotent stem cell-derived hepatic cell lines as a new model for host interaction with hepatitis B virus

Figure 3

iPS-HPCs respond to IFN stimulation.

(a) Immunoblot analysis of STAT1, phosphorylated STAT1, STAT2 and IRF9 in iPS-HPCs treated with IFN-α. (b) Quantitative RT-PCR analysis of IFN-stimulated genes (MxA, ISG15 and PKR) in iPS-HPCs and HepG2-NTCP treated with or without IFN-α for 48 hours. The y-axis represents the ratio between the copy number of each cell type with IFN-α and the mean of each cell type without IFN-α. (c) Schema of the schedule for treatment against iPS-HPCs transfected with plasmids expressing the HBV DNA genome. (d) The expression of intracellular HBV DNA and the secretion of HBsAg from iPS-HPCs transfected with plasmids expressing the HBV DNA genome. The effect of IFN-α treatment on the secretion of HBsAg from iPS-HPCs was blocked by the addition of Janus kinase inhibitor I (JAKi) in a dose-dependent manner. **p < 0.01.

Back to article page