Figure 1: MS suppresses the production of pro-inflammatory cytokines in LPS stimulated peritoneal macrophages (PMs) and bone marrow-derived macrophages (BMDMs) of mice. | Scientific Reports

Figure 1: MS suppresses the production of pro-inflammatory cytokines in LPS stimulated peritoneal macrophages (PMs) and bone marrow-derived macrophages (BMDMs) of mice.

From: Methane limit LPS-induced NF-κB/MAPKs signal in macrophages and suppress immune response in mice by enhancing PI3K/AKT/GSK-3β-mediated IL-10 expression

Figure 1

PMs (A,B) or BMDMs (C,D) were treated with LPS (100 ng/ml) for 6 h and the media were collected and analysed for the TNF-α and IL-6 level by ELISAs (A,C). For the protein expression analysis, MS was added 30 min before or after LPS stimulation at the indicated concentrations. For the mRNA expression analysis, MS was added 10 μl/ml for pre-treatment and post-treatment. The mRNA expression was quantified by Q-PCR at the indicated time points (B,D). Data are expressed as the mean ± SD of at least three independent experiments. Unpaired Student’s t-test, *P < 0.05; **P < 0.01; ***P < 0.001 compared with the LPS-induced control.

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