Figure 5: MS promotes IL-10 production in macrophages and in vivo. | Scientific Reports

Figure 5: MS promotes IL-10 production in macrophages and in vivo.

From: Methane limit LPS-induced NF-κB/MAPKs signal in macrophages and suppress immune response in mice by enhancing PI3K/AKT/GSK-3β-mediated IL-10 expression

Figure 5

(A,B) Peritoneal macrophages were treated with 100 ng/ml LPS and MS (10 μl/ml) was given 30 min after LPS stimulation. The IL-10 levels were determined by Q-PCR (A) and ELISA (B) at the indicated time points. (C) ELISA of the serum IL-10 levels 6 h after LPS challenge in mice (intraperitoneal injection of LPS, 10 mg/kg body weight). For MS post-treatment, the mice were given MS (16 ml/kg body weight) or saline i.p. 30 min after LPS injection (n = 6). (D) The serum IL-10 levels 5 h after i.p. infection with 1 × 107 E. coli 0111:B4 followed by i.p.-injected MS (16 ml/kg body weight) or saline 30 min after E. coli challenge (n = 4). (E) The serum IL-10 level 6 h after CLP or in combination with MS post-treatment (16 ml/kg body weight) (n = 4). (F) ELISA of the serum IL-10 levels of MS and control mice (n = 5) at 6 h on day 1 after feeding with 3% DSS and i.p. injection with MS (16 ml/kg body weight). Data are presented as the mean ± SD of at least two repeats. *P < 0.05; **P < 0.01; ***P < 0.001 (Student’s t-test, analysis of variance, compared with the NS control).

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