Figure 4

Effect of LeftyA on actin polymerization in Ishikawa cells in absence or presence of Rac1 inhibitor.

(a) Representative original Western blot of soluble G-actin over F-actin in human endometrial cancer Ishikawa cells after a 2 hour treatment without and with LeftyA (25 ng/ml) in the absence and presence of the Rac1 inhibitor NSC23766 trihydrochloride (100 μM). (b) Arithmetic means ± SEM (n = 3; arbitrary units) of soluble G-actin over F-actin ratio in Ishikawa cells after a 2 hour treatment without and with LeftyA (25 ng/ml) in the absence and presence of the Rac1 inhibitor NSC23766 trihydrochloride (100 μM). (c) Representative original histogram of DNase1 (G-actin; Upper) and Phalloidin (F-actin; Lower) binding in Ishikawa cells after a 2 hour treatment without and with LeftyA (25 ng/ml) in the absence and presence of the Rac1 inhibitor NSC23766 trihydrochloride (100 μM). (d) Arithmetic means ± SEM (n = 5 arbitrary units) of G-actin over F-actin ratio in Ishikawa cells after a 2 hour treatment without and with LeftyA (25 ng/ml) in the absence and presence of the Rac1 inhibitor NSC23766 trihydrochloride (100 μM) *P < 0.05; **P < 0.01; ***P < 0.001 using Student’s t-test.