Figure 5: TGFβi impact on osteogenesis.

(a) In silico changes in bone area (μm2) were predicted at 25-day intervals over a 250 day period. (b,c) The effect of TGFβ inhibition on the average number of proliferative and apoptotic osteoblasts per day in silico. (d) μCT analysis of bone volume (arrow) to total volume (BV:TV) in control and TGFβ inhibitor treated bones. (e,f) Histomorphometry analysis of osteogenesis (e) and number of bone rimming osteoblasts (arrows; f). Asterisks denote statistical significance (*p < 0.05; **p < 0.005; ***p < 0.0001).