Figure 5: Overexpression of PTX3 induces cell proliferation, migration and invasion in human cervical HeLa cells.

(a) HeLa cells were transfected with 3 μg of PTX3 plasmid and vector only (Neo) by lipofectamine agents. Cell viability was detected for 24, 48 and 72 h by MTT assay. (b) The migration and invasion of PTX3 and Neo cells were measured by in vitro migration and invasion assay. The non-migratory cells were removed and migrating cells were fixed and stained by crystal violet staining. The images were examined using a microscope (upper panel). (c) The expression levels of cell cycle related proteins (p21, p27, cyclin B, p-cdc2, cdc2, p-cdc25c, cdc25c) and invasive-related proteins (MMP2, MMP9, and uPA) in PTX3 over-expressing HeLa cells by western blotting (d) HeLa cell lines were treated with 50 ng/ml PTX3 for 48 h. Total lysates of cells were analyzed by western blotting with antibodies against PTX3 and b-actin protein (e) Cell viability were detected for 24, 48 and 72 h by MTT assay. (f) The migrates and invasive properties of HeLa cells were treated with 50 ng/ml PTX3 for 48 h, and examined using in vitro migration and invasion assay. Values represent the mean ± S.E. of three determinations.