Figure 2
From: Efficient Inhibition of Hepatitis B Virus Infection by a preS1-binding Peptide
Mapping of the main binding site of 4B10 in preS1.
(A) Schematic presentation of mutant preS1/2-48myr peptides. IC50, concentration for achieving 50% inhibition of the interaction between preS1/2-48myr-FITC and 4B10, calculated based on the data plotted in (B). The five-amino acid deletions were depicted as lines and the myristoyl groups as open circles. Places where alanine was substituted were indicated. (B) Peptide competition assays. Each mutated preS1/2-48myr peptide was used as a competitor of preS1/2-48myr-FITC to bind to immobilized N-biotinylated 4B10. The fluorescence intensity was determined and normalized to that from the control competitorless wells. The numbers on the x axis represent the molar ratios of competitor versus preS1/2-48myr-FITC. BSA, bovine serum albumin. (C) 4B10-binding activities of alanine substituted preS1/2-48myr peptides. Wild type or alanine substituted preS1/2-48myr-FITC was incubated with immobilized N-biotinylated 4B10. The fluorescence intensity was determined and relative binding activity was calculated, with the activity of the wild type peptide taken as 1.0. *p < 0.05; ***p < 0.001; ns, not significant.
