Figure 3
From: Efficient Inhibition of Hepatitis B Virus Infection by a preS1-binding Peptide
4B10 inhibited HBV attachment to hepatic cells.
(A) 4B10-FITC and LA-20-FITC did not bind to PMH. Fluorescence-activated cell sorting detected the binding of preS1/2-48myr-FITC but not 4B10-FITC and LA-20-FITC to PMH. (B) 4B10 inhibited the binding of preS1/2-48myr-FITC to PMH. PMH-bound preS1/2-48myr-FITC peptides were visualized via confocal fluorescent microscopy. (C,D) 4B10 inhibited HBV attachment to PMH. (C) HBV virions bound to PMH were quantified via real-time PCR. (D) Immunofluorescent staining of cell-bound HBsAg. (E) 4B10 and LA-20 had no cytotoxic effect on PMH. Cell viability was measured using CCK-8 test.
