Figure 3
From: Rapid and label-free microfluidic neutrophil purification and phenotyping in diabetes mellitus
Neutrophil rolling on E-selectin.
(A) Image overlay (60× magnification) from captured cell rolling videos highlight distinct differences in rolling trajectories between healthy and TNF-α-treated neutrophils. (B) Cumulative frequency curves of neutrophil rolling speed for healthy, glucose-treated (30 mM), TNF-α-treated (10 ng/mL) and PMA-treated (1 μM) neutrophils. The rolling speeds for all treated groups (~50–200 cells in each group) were significantly different as compared to healthy neutrophils (P < 0.0001). Representative flow cytometric analysis of (C) PSGL-1 and (D) CD11b expression on healthy and treated neutrophils. Cells were incubated with mouse APC anti-human PSGL-1 IgG2a/mouse APC IgG2a isotype and FITC mouse anti-human CD11b (activated) IgG1/FITC mouse IgG1 isotype.
