Figure 5

Neutrophil rolling in T2DM patients.

(A) High purity of DFF-sorted neutrophils (~90%) from T2DM patients. Mean ± s.d. from n = 6 *P < 0.05, **P < 0.01. (B) Frequency distribution and (C) average rolling speed of neutrophils from healthy (n = 16) and T2DM patients (n = 16) in microchannel functionalized with E-selectin. Mean ± s.e.m. *P < 0.01. Representative images overlay of neutrophil rolling trajectories in healthy and T2DM patients. Correlation of average rolling speed with neutrophil (D) intracellular ROS level and (E) PSGL-1 expression. (F) Neutrophil circularity of healthy and T2DM patients. Mean ± s.d. n = 300 cells (from 5 healthy controls) and 500 cells (from 7 T2DM patients). ***P < 0.001. Inset plot shows frequency distribution of neutrophil circularity. Representative brightfield images (60× magnification) illustrating neutrophil shape differences from healthy (grey box) and T2DM (black box) patients.