Table 1 Cytokines and chemokines production by monocytes, mDCs and pDCs.

Cell type	Ligand	TNF-a	IL-6	IL-1β	IL-10	IL-12	CXCL9	CXCL10
monocyte	NS	45	61	124	34	41	68	680
	cl097	8251	11884	5392	153	93	183	970
	DOTAP	54	112	133	34	39	73	817
	HCVL1	9344	12383	4927	75	157	408	1189
	HCVL1mut	48	961	196	41	37	66	681
	HCVL4	7252	12267	2803	205	66	360	1092
	HCVL4mut	53	205	131	65	44	62	679
	HCVL8	12483	12704	5540	223	176	355	1135
	HCVL8mut	276	3780	333	57	39	89	877
mDC	NS	287	4266	382	84	48	96	716
	cl097	9397	10443	6335	188	400	1122	877
	DOTAP	120	317	282	51	37	111	726
	HCVL1	3638	6354	3355	153	179	1427	1587
	HCVL1mut	59	391	302	55	37	92	680
	HCVL4	4060	8545	2115	80	200	1527	1483
	HCVL4mut	41	352	215	51	39	128	760
	HCVL8	6383	8528	3391	138	329	1432	1666
	HCVL8mut	79	433	261	53	43	91	743
pDC	NS	37	564	129	38	37	1087	1177
	cl097	1693	2257	224	60	74	13623	5653
	DOTAP	50	43	110	30	42	304	1899
	HCVL1	2155	2047	202	56	61	8349	6370
	HCVL1mut	40	47	109	33	41	362	2473
	HCVL4	1883	2370	183	68	48	9793	5862
	HCVL4mut	45	52	110	33	45	786	1662
	HCVL8	5476	2350	170	59	87	9309	6975
	HCVL8mut	58	55	109	35	38	328	2051

Monocytes, mDCs and pDCs (10⁵/200 μl) were isolated from 3 healthy donors by negative selection using magnetic beads and stimulated with the indicated HCV ssRNA sequences (7.5 μg/ml). The supernatants were harvested at 24 h post-stimulation. Levels of cytokines TNF-α, IL-6, IL-1β, IL-10, IL-12p70, and chemokines CXCL9, CXCL10 were measured in the cell culture supernatant by CBA. The U to A mutated forms of HCVL1, HCVL4 and HCVL8 were used as negative controls. Values represent mean of results generated with cells from 3 different donors.

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