Figure 2 | Scientific Reports

Figure 2

From: Cosmid based mutagenesis causes genetic instability in Streptomyces coelicolor, as shown by targeting of the lipoprotein signal peptidase gene

Figure 2

IS21 insertion into scr6809.

The sco6811-08 region of the S. coelicolor M145 genome contains 4 genes (sco6811 (Purple), sco6810 (green), sco6809 (yellow) and sco6809 (blue) and a putative sRNA scr6809 (large red) along with 3 putative promoters (broken arrows). Representations of the WT loci (a) and that sequenced from BJT1004 (b) indicate where an IS21 element (sco6393 and sco6394) has inserted within scr6809. PCR verification of the IS21 insertion with primers JM0093 and JM0094 (small red arrows) was carried out (c) using M145 (lane 1), BJ1001 (lane 2) and BJT1004 (lane 3) genomic DNA. Lanes marked L contain the size ladders (Invitrogen 1 kb plus DNA ladder), lane 1 contains the PCR product using wild-type M145 DNA (514 bp), lane 2 contains the PCR product using ∆lsp strain BJT1001 DNA and lane 3 contains the PCR product using genomic DNA from the cis complemented ∆lsp strain BJT1004 (both 2884 bp.

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