Figure 5: Double reciprocal plots of the kinetic study of Dcsbis protein in absent and present of c-di-GMP. | Scientific Reports

Figure 5: Double reciprocal plots of the kinetic study of Dcsbis protein in absent and present of c-di-GMP.

From: Dcsbis (PA2771) from Pseudomonas aeruginosa is a highly active diguanylate cyclase with unique activity regulation

Figure 5

This analysis was done by measuring three independent sets of experiments with 5 substrate concentrations (128 μM, 150 μM, 190 μM, 333 μM, 512 μM). The plots are generated by plotting 1/V as a function 1/[S]. The intercept on the vertical axis is 1/Vmax, and the intercept on the horizontal axis is −1/KM.

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