Figure 2
(A) The effect of the streptavidin coating. Experimental conditions: the capture probe, LPS, the detection probe, H1/H2 and SA-HRP were 100 nM, 10 μg/mL, 500 nM/500 nM and 500 ng/mL, respectively. (B) The effect of the capture probe: the streptavidin coating, LPS, the detection probe, H1/H2 and SA-HRP were 2 μg/mL, 10 μg/mL, 100 nM, 500 nM/500 nM and 500 ng/mL, respectively. (C) The effects of detection probe sequence. Experimental conditions: the streptavidin coating, the capture probe, LPS and SA-HRP were 2 μg/mL, 100 nM, 5 μg/mL and 500 ng/mL, respectively. (D) The effects of SA-HRP. Experimental conditions: the streptavidin coating, the capture probe, LPS, the detection probe and H1/H2 were 2 μg/mL, 100 nM, 0.5 μg/mL, 50 nM and 250 nM/250 nM.
