Figure 3
(A) The effects of reaction time. Experimental conditions: (1–10) the detection probe andH1/H2 were 50 nM and 250 nM/250 nM, respectively. (1, 2) reaction time was 0.5 h, (3, 4) reaction time was 1 h, (5, 6) reaction time was 2 h, (7, 8) reaction time was 3 h, (9, 10) reaction time was 4 h; (11, 12) 50 nM of the detection probe; (13, 14) 250 nM of H1; (15, 16) 250 nM of H2; (17, 18) 250 nM of H1 and 250 nM of H2, reaction time was 4 h. (B) The effects of reaction temperature. Experimental conditions: (1, 2) 50 nM of the detection probe; (3, 4) 250 nM of H1; (5, 6) 250 nM of H2; (7, 8, 11, 12, 15, 16, 19, 20) 250 nM of H1 and 250 nM of H2; (9, 10, 13, 14, 17, 18, 21, 22) the detection probe and H1/H2, were 50 nM, 250 nM/250 nM. (7–10) reaction temperature was 37 °C, (11–14) reaction temperature was 25 °C, (15–18) reaction temperature was 15 °C, (19–22) reaction temperature was 10 °C.
