Figure 4

The optical density value vs. different detection methods.
Experimental conditions of HCR: capture probe, detection probe, H1/H2 and SA-HRP were 100 nM, 50 nM, 250 nM/250 nM and 500 ng/mL, respectively; Experimental conditions of Non-HCR: capture probe, detection probe and SA-HRP were 100 nM, 50 nM and 500 ng/mL, respectively; the reaction temperature was 37 °C. The detection procedure was carried out as described in the Experimental section.