Figure 2
From: Host Double Strand Break Repair Generates HIV-1 Strains Resistant to CRISPR/Cas9
HIV-1 strain NL4-3 replication is delayed in human CD4+ T cell lines expressing CRISPR.
Wild type human CD4+ T cell line SupT1 and derivatives expressing Cas9 and multiple gRNAs targeting HIV-1 were infected with strain NL4-3. (Left) Culture supernatants were assayed for the release of p24 as a measure of viral replication. All gRNAs suppress viral replication compared to wild type cells with no gRNA (WT). (Right) Cell viability was measured by trypan blue exclusion. As viral replication increases, cellular viability decreases. Error bars indicate the standard deviation between three separate infections.
