Figure 5

CRISPR resistant strains have indel mutations at the double strand break.

Human CD4+ T cell lines expressing Cas9 and multiple gRNAs were infected with (a–f) the strain NL4-3 or (g–l) the strain R7. CRISPR resistant strains were characterized for indel mutations 5 weeks after infection. All resistant strains display indels. R7 infection of gRNA-TATA₁ cells is a negative control and displays the background level of indels. The 23 bp gRNA targeted sequence is shown. Red dotted lines indicate the site of Cas9 cleavage. Black bars indicate a deleted base. Light gray bars indicate insertions. Yellow boxes highlight bases that encode a bulge or loop in an RNA structure.