Figure 7
From: Host Double Strand Break Repair Generates HIV-1 Strains Resistant to CRISPR/Cas9
Base substitutions of CRISPR resistant strains present at variable positions of the gRNA homology site.
CRISPR cell lines were infected with (a–f) strain NL4-3 or (g–l) strain R7. CRISPR resistant strains were characterized for transition (blue bars) and transversion (red bars) mutations. The 23 bp gRNA targeted sequence is shown. Red dotted lines indicate the site of Cas9 cleavage. Yellow boxes highlight bases that give rise to unpaired nucleotides of an RNA structure.
